4/15/2024 0 Comments What gender does scids effect![]() These functional SRC subpopulations are distinguished by the period between cell injection and analysis of their progeny (6-8 weeks and 12-14 weeks for ST- and LT-SRC, respectively) or by using serial transplantations (limited primary or serial (secondary and more) engraftment capacity for ST- and LT-SRC, respectively). The immuno-deficient models are able to evidence the functional heterogeneity of HSC population that contains short term (ST-) and long term (LT-) SRC. The recently developed NOG (NOD/Shi- scid/IL-2Rγ null) and NSG (NOD.Cg- Prkdc scid Il2rg tm1Wjl/SzJ) mouse strains are now considered as the most efficient model for studying human HSC, commonly named “Scid Repopulating Cells” (SRC). Afterwards, NOD/SCID model, exhibiting a more severe immune deficit due to the combined alteration of NK cells, complement and macrophages, was used thereafter. To address human HSC, CB17-SCID and NIH Beige-Nude-Xid mice strains were used up to 1995. This test is based on the unique functional capacity of HSC to durably engraft recipients in which they self-renew, expand and give rise to a progeny of more mature hematopoietic cells. In vivo xenotransplantation assays are thus mandatory to reveal and quantify human HSC. Indeed, even though human CD34 +CD38 -/lowCD90 +CD45RA - hematopoietic cell population is very enriched in stem cells, it still contains a non-negligible proportion of HP. Hematopoietic stem cells (HSC) cannot be distinguished from Hematopoietic Progenitors (HP) by phenotypic and/or molecular analysis. However, this does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have read the journal’s policy and declare the following interest: co-author Dr Zoran IVANOVIC is a PLOS ONE Editorial Board Member. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: The source of funding for this work is the French Blood Institute. Received: JAccepted: JPublished: September 17, 2013Ĭopyright: © 2013 Chevaleyre et al. PLoS ONE 8(9):Įditor: Graca Almeida-Porada, Wake Forest Institute for Regenerative Medicine, United States of America (2013) Busulfan Administration Flexibility Increases the Applicability of Scid Repopulating Cell Assay in NSG Mouse Model. Citation: Chevaleyre J, Duchez P, Rodriguez L, Vlaski M, Villacreces A, Conrad-Lapostolle V, et al.
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